2-(2-((tert-butoxycarbonyl)amino)thiazol-4-yl)-2-oxoacetic acid 13C, 15N2

2-(2-((tert-butoxycarbonyl)amino)thiazol-4-yl)-2-oxoacetic acid 13C, 15N2 is a complex chemical compound that is commonly used in biological research. It is a labeled form of 2-(2-((tert-butoxycarbonyl)amino)thiazol-4-yl)-2-oxoacetic acid, which means it contains isotopes of carbon and nitrogen. These isotopes are used to track the movement and behavior of the compound in biological systems. The primary use of this compound is in the study of metabolic pathways and protein synthesis. It is commonly used in experiments involving stable isotope labeling by amino acids in cell culture (SILAC), which is a technique used to study protein expression and turnover rates. The labeled compound is added to cell cultures, allowing researchers to track the behavior of the labeled proteins over time. This information can then be used to understand how proteins are synthesized and metabolized within the cell. Chemically, 2-(2-((tert-butoxycarbonyl)amino)thiazol-4-yl)-2-oxoacetic acid 13C, 15N2 is a thiazole derivative that contains a carbonyl group and a carboxylic acid group. It has a molecular weight of 289.3 g/mol and a melting point of 174-177°C. The labeled isotopes of carbon and nitrogen do not significantly alter the chemical properties of the compound, but they do allow for more precise tracking of the compound in biological systems.