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[Cyano-13C]-3-Cyanobenzoic acid, also known as 13C-labeled 3-cyanobenzoic acid, is a chemical compound that contains a stable isotope of carbon, 13C. This isotope is used in various research applications, including nuclear magnetic resonance (NMR) spectroscopy, mass spectrometry, and metabolic labeling studies.
In NMR spectroscopy, [Cyano-13C]-3-Cyanobenzoic acid is used as a reference standard for chemical shift calibration. The carbon-13 atom in the cyano group of the molecule has a different chemical shift than the other carbons in the molecule, allowing for precise calibration of NMR instruments and accurate identification of unknown compounds.
In mass spectrometry, [Cyano-13C]-3-Cyanobenzoic acid is used as a matrix for matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. The labeled compound helps improve the accuracy and resolution of mass spectrometry measurements, allowing for more precise identification of proteins, peptides, and other biomolecules.
In metabolic labeling studies, [Cyano-13C]-3-Cyanobenzoic acid is used as a tracer to study the metabolic pathways of various organisms. The labeled compound is incorporated into biomolecules, allowing researchers to track the movement of carbon atoms through metabolic pathways and gain insight into cellular metabolism.
Overall, [Cyano-13C]-3-Cyanobenzoic acid is a valuable tool for researchers in a variety of fields, providing precise and accurate measurements in various analytical techniques.
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