N-(4-Hydroxyphenyl)acetamide 13C6

[13C6]-N-(4-Hydroxyphenyl)acetamide is a stable isotope labeled version of N-(4-Hydroxyphenyl)acetamide, which is also known as paracetamol or acetaminophen. It is a commonly used analgesic and antipyretic drug that is used to relieve pain and reduce fever. [13C6]-N-(4-Hydroxyphenyl)acetamide is used in pharmacokinetic studies to determine the metabolism and excretion of acetaminophen in the body. The chemical formula of [13C6]-N-(4-Hydroxyphenyl)acetamide is C8H8D6NO2 and its molecular weight is 167.20 g/mol. It is a white to off-white crystalline powder that is soluble in water and ethanol. The stable isotope labeling of [13C6]-N-(4-Hydroxyphenyl)acetamide provides a unique tool to track the pharmacokinetics of acetaminophen in the body. The metabolism of acetaminophen involves the oxidation of the phenolic ring by hepatic cytochrome P450 enzymes, which leads to the formation of a reactive metabolite called N-acetyl-p-benzoquinone imine (NAPQI). NAPQI can cause liver damage when it accumulates in the liver, but it is usually detoxified by conjugation with glutathione. The use of [13C6]-N-(4-Hydroxyphenyl)acetamide allows researchers to track the formation and elimination of NAPQI in vivo, which is important for understanding the toxicity of acetaminophen and for developing strategies to prevent or treat acetaminophen-induced liver injury.