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[1-15N]-Adenine, also known as 1-^15N-adenine or ^15N-labeled adenine, is a stable isotope-labeled compound commonly used in various biochemical and biophysical studies. Adenine is one of the four nucleotide bases that form the building blocks of DNA and RNA, and ^15N-labeled adenine allows researchers to track and study the behavior of this important molecule in living systems.
^15N-labeled adenine is typically used in NMR spectroscopy and mass spectrometry experiments to study the structure and dynamics of nucleic acids and nucleic acid-protein complexes. The labeling with ^15N allows researchers to distinguish the isotopically labeled molecule from the natural abundance of nitrogen in biological samples, improving the sensitivity and accuracy of the experiments.
In addition to its usage in research, ^15N-labeled adenine also has potential applications in medical diagnostics and drug discovery. Adenine derivatives are used in a variety of drugs, including antiviral and anticancer agents, and the labeled compound can be used to study the distribution and metabolism of these drugs in vivo.
Chemically, ^15N-labeled adenine has the same structure as natural adenine, with the only difference being the substitution of one of the nitrogen atoms with the stable isotope ^15N. The compound is synthesized using specialized techniques that ensure high purity and isotopic enrichment, making it a valuable tool for biological and biomedical researchers.
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