[13C6]-6-Hydroxychlorzoxazone

[13C6]-6-Hydroxychlorzoxazone is a stable isotope-labelled derivative of chlorzoxazone, which is a centrally acting muscle relaxant used in the treatment of musculoskeletal pain and spasms. It is used as a metabolic probe to study the activity of cytochrome P450 (CYP) enzymes, particularly CYP2E1, in vitro and in vivo. The compound is often used in drug metabolism and pharmacokinetic studies, as it can be easily detected and quantified using mass spectrometry. The use of stable isotope-labelled compounds in these studies helps to improve the accuracy and precision of the results, as well as reducing the risk of false positives. The chemical structure of [13C6]-6-Hydroxychlorzoxazone is similar to that of chlorzoxazone, with the addition of six carbon-13 atoms in the molecule. This stable isotope labelling has no significant effect on the pharmacological activity of the compound, but it does allow for accurate measurement of the metabolic pathways and elimination of the drug. In conclusion, [13C6]-6-Hydroxychlorzoxazone is a valuable tool for studying drug metabolism and pharmacokinetics. Its stable isotope labelling allows for accurate measurement of the activity of CYP enzymes, which is important for the development of new drugs and the understanding of drug-drug interactions.