Kifunensine 13C2, 15N

[13C2, 15N]-Kifunensine is a modified form of kifunensine, a potent inhibitor of the enzyme alpha-mannosidase I. This enzyme is involved in the processing of glycoproteins, which play crucial roles in various biological processes such as cell-cell recognition, immune response, and protein folding. By inhibiting alpha-mannosidase I, [13C2, 15N]-Kifunensine can disrupt glycoprotein processing, leading to the accumulation of misfolded or incomplete glycoproteins in the endoplasmic reticulum (ER) of cells. [13C2, 15N]-Kifunensine is commonly used in research to study glycoprotein processing and ER stress response. Specifically, it is used to label glycoproteins with stable isotopes, allowing for their identification and quantification using mass spectrometry. This technique, known as stable isotope labeling with amino acids in cell culture (SILAC), enables researchers to compare the glycoprotein profiles of cells treated with [13C2, 15N]-Kifunensine to those of untreated cells, providing insights into the effects of glycoprotein processing inhibition. In terms of chemical information, [13C2, 15N]-Kifunensine has a molecular formula of C12H15D2N2O6 and a molecular weight of 317.3 g/mol. It is a stable isotope-labeled compound, meaning that it contains two additional heavy isotopes (13C and 15N) compared to the natural abundance of these isotopes in standard kifunensine. These isotopes do not affect the inhibitory activity of [13C2, 15N]-Kifunensine, but allow for its detection and quantification in complex biological samples.