4β-Hydroxycholesterol-[2,2,3,4,5-d5]

4β-Hydroxycholesterol-[2,2,3,4,5-d4] is a chemical compound that is used as an analytical standard for the quantification of 4β-hydroxycholesterol in biological samples. This compound is labeled with five deuterium atoms, which makes it useful for stable isotope dilution mass spectrometry (SIDMS) analysis. SIDMS is a highly sensitive analytical technique that is used to measure trace amounts of metabolites in biological fluids like blood, urine, and cerebrospinal fluid. 4β-Hydroxycholesterol is a cholesterol metabolite that is produced in the liver via the cytochrome P450 enzyme system. It is a marker of cholesterol turnover and is used as a biomarker for assessing cholesterol metabolism and predicting cardiovascular disease risk. The deuterium-labeled form of 4β-Hydroxycholesterol is used to accurately quantify the levels of this metabolite in biological samples. The chemical formula of 4β-Hydroxycholesterol-[2,2,3,4,5-d4] is C27H39D4O2, and its molecular weight is 409.7 g/mol. It is a white to off-white powder that is stable under normal conditions. This compound should be stored at -20°C in a dry and well-ventilated area. It is important to handle this compound with care and follow all safety protocols when working with it.