N'-Nitrosonornicotine-2,4,5,6-d4(pyridine-d4)

N'-Nitrosonornicotine-2,4,5,6-d4(pyridine-d4), also known as NNK-d4, is a deuterium-labeled derivative of N'-Nitrosonornicotine (NNK), which is a tobacco-specific nitrosamine (TSNA) and a potent carcinogen. NNK-d4 is used as an internal standard in various analytical methods to accurately quantify NNK levels in tobacco products and biological samples. NNK is formed during the curing and processing of tobacco leaves and is present in cigarette smoke. It is known to cause lung cancer, pancreatic cancer, and other types of cancers. As a result, NNK has been classified as a Group 1 carcinogen by the International Agency for Research on Cancer (IARC). NNK-d4 is synthesized using deuterated pyridine and labeled with deuterium atoms at positions 2, 4, 5, and 6. Deuterium labeling is used to improve the accuracy and precision of analytical methods by reducing the interference from other compounds and improving the sensitivity of detection. In conclusion, NNK-d4 is a valuable tool in the analysis of NNK in tobacco products and biological samples. Its use enables accurate quantification of NNK levels, which is important for understanding the health risks associated with tobacco use and for developing effective tobacco control policies.