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2-Deoxy-2-acetamido-D-glucose-6,6-d2, also known as N-acetylglucosamine-6,6-d2 or 2-DAG, is a stable isotope-labeled form of N-acetylglucosamine (GlcNAc), a monosaccharide derivative of glucose. It is commonly used in biomedical research as a metabolic tracer to study the synthesis, degradation, and signaling pathways of complex carbohydrates and glycosylation.
2-DAG is a white crystalline powder that is soluble in water and polar solvents. It is synthesized by introducing deuterium isotopes into the acetamido group of GlcNAc via chemical or enzymatic methods. The deuterium-labeled compound has similar physicochemical properties as the natural compound, but with a higher mass, which allows for accurate detection and quantification by mass spectrometry.
In biological systems, GlcNAc is involved in various cellular processes, including cell growth, differentiation, inflammation, and host-pathogen interactions. By using 2-DAG as a metabolic tracer, researchers can track the fate of GlcNAc in vivo and in vitro, and gain insights into the dynamics of glycosylation in different biological systems.
Overall, 2-Deoxy-2-acetamido-D-glucose-6,6-d2 is a valuable tool for investigating the role of GlcNAc in health and disease, and for developing new diagnostic and therapeutic strategies targeting glycosylation-related pathways.
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