1H-​Imidazole-​4-​d, 2,​5-​dimethyl- (9CI)

1H-Imidazole-4-d, 2,5-dimethyl- (9CI) is a chemical compound that is commonly used in biochemical research as a stable isotope-labeled internal standard. It is a deuterated form of the heterocyclic compound imidazole, which contains two nitrogen atoms in a five-membered ring. The 2,5-dimethyl substituents on the imidazole ring have a significant impact on the compound's physical and chemical properties, including its solubility and boiling point. The compound's main application is in the field of mass spectrometry, where it serves as a reference standard for the measurement of analytes in biological samples. The use of 1H-Imidazole-4-d, 2,5-dimethyl- (9CI) as an internal standard allows for accurate quantification of analytes by correcting for variations in sample preparation and instrument performance. Additionally, it is used in the synthesis of other deuterated compounds for use as internal standards. From a chemical standpoint, 1H-Imidazole-4-d, 2,5-dimethyl- (9CI) is a stable compound that is not reactive under normal laboratory conditions. It has a molecular formula of C5H6D2N2 and a molecular weight of 96.15 g/mol. The compound is soluble in most organic solvents and has a boiling point of 218-220°C at atmospheric pressure. Its stability and solubility make it an ideal internal standard for use in mass spectrometry applications.