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[1'-13C]uridine 5'-monophosphate (disodium salt) is a stable isotope-labeled nucleotide that has a 13C atom at the 1' position of the uracil ring. This compound is commonly used in various biochemical and biophysical studies, particularly in the field of nucleic acids research.
One of the primary uses of [1'-13C]uridine 5'-monophosphate (disodium salt) is to study the structure, dynamics, and function of RNA and DNA molecules. The labeled nucleotide can be incorporated into RNA or DNA sequences using standard chemical synthesis or enzymatic methods. This incorporation allows researchers to track the movement and interactions of specific nucleotides within the nucleic acid backbone, providing valuable insights into the mechanisms of RNA and DNA folding, stability, and catalysis.
In addition to its use in nucleic acid research, [1'-13C]uridine 5'-monophosphate (disodium salt) can also be used in metabolic studies. The labeled nucleotide can be administered to cells or organisms, and its incorporation into nucleic acids can be monitored to investigate nucleotide turnover rates, RNA and DNA synthesis, and other metabolic processes.
From a chemical perspective, [1'-13C]uridine 5'-monophosphate (disodium salt) has a molecular formula of C9H11N2O9PNa2 and a molecular weight of 400.14 g/mol. It is a white to off-white powder that is soluble in water and slightly soluble in ethanol. The labeled nucleotide is stable under normal storage conditions and can be stored at -20°C for long-term use.
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