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[1'-D]uridine is a modified form of uridine, a nucleoside that is an essential building block for RNA synthesis. The modification involves replacing the hydrogen atom on the 1' carbon of the ribose moiety with a deuterium atom. This modification can have significant effects on the biological activity and stability of the molecule.
[1'-D]uridine has been used extensively in research as a tracer molecule for studying RNA metabolism and turnover. Since it is a stable isotope, it can be easily tracked and quantified using mass spectrometry techniques. Additionally, the deuterium substitution can alter the binding properties of the molecule, allowing for the study of specific interactions with RNA-binding proteins and enzymes.
In terms of chemical properties, [1'-D]uridine has a molecular weight of 247.25 g/mol and a melting point of 167-169°C. It is soluble in water and slightly soluble in ethanol and methanol. The deuterium substitution does not significantly alter the physical properties of the molecule, but it can affect the reactivity and stability of the molecule in certain conditions.
Overall, [1'-D]uridine is a valuable tool for studying RNA biology and can provide insights into the mechanisms underlying various diseases and disorders. Its stable isotope labeling and altered binding properties make it an attractive option for researchers seeking to understand the complex interactions between RNA and other biomolecules.
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