[1,3-15N2]-2'-deoxyuridine

[1,3-15N2]-2'-deoxyuridine is a stable isotope labeled compound that is commonly used in various biochemical assays to study the metabolism and biosynthesis of DNA. It is chemically synthesized by introducing stable isotopes of nitrogen (15N) into the nucleotide base of 2'-deoxyuridine. The resulting compound, [1,3-15N2]-2'-deoxyuridine, is a highly purified and stable compound that can be used in a variety of research applications. One of the primary uses of [1,3-15N2]-2'-deoxyuridine is in metabolic labeling experiments. In these experiments, cells are grown in the presence of the labeled compound, and the incorporation of the labeled nucleotide into DNA can be monitored over time. This allows researchers to track the synthesis of new DNA strands and study the kinetics of DNA replication. In addition to its use in metabolic labeling, [1,3-15N2]-2'-deoxyuridine can also be used as a tracer in studies of nucleotide metabolism. By introducing the labeled compound into cells or tissues, researchers can track the fate of the nucleotide and study the various pathways involved in its metabolism. Overall, [1,3-15N2]-2'-deoxyuridine is a useful tool for studying DNA metabolism and biosynthesis. Its stable isotope labeling allows for precise and accurate measurements, making it an invaluable reagent for researchers in the fields of biochemistry, genetics, and molecular biology.