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Trans-7,8-Dihydroxy-7,8-dihydrobenzo[a]pyrene-d8 is a chemical compound that is widely used in biochemical research as a stable isotope labeled internal standard for the quantitative analysis of benzo[a]pyrene and its metabolites in biological samples. It is a deuterated analog of trans-7,8-Dihydroxy-7,8-dihydrobenzo[a]pyrene, which is a carcinogenic compound found in cigarette smoke, automobile exhaust, grilled meats, and other sources of combustion.
Trans-7,8-Dihydroxy-7,8-dihydrobenzo[a]pyrene-d8 has a molecular formula of C20D8H10O2 and a molecular weight of 298.37 g/mol. It is a white to off-white powder that is stable under normal conditions. Its melting point is not available in the literature, but it is soluble in organic solvents such as methanol, acetonitrile, and dimethyl sulfoxide.
The primary use of trans-7,8-Dihydroxy-7,8-dihydrobenzo[a]pyrene-d8 is in mass spectrometric analysis, where it is used as an internal standard for the quantitative determination of benzo[a]pyrene and its metabolites in biological samples such as blood, urine, and tissues. The inclusion of the deuterium atoms in the molecule allows for accurate measurement of the compound's concentration and helps to compensate for variations in sample preparation and analysis. It is also used in other analytical methods such as high-performance liquid chromatography and gas chromatography.
In conclusion, trans-7,8-Dihydroxy-7,8-dihydrobenzo[a]pyrene-d8 is a key compound in biochemical research that is used to detect and quantify benzo[a]pyrene and its metabolites in biological samples. Its stable isotopic nature and chemical properties make it an ideal internal standard for mass spectrometry and other analytical techniques.
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