5-Fluorouracil-6-d1

5-Fluorouracil-6-d1 (5-FU-6-d1) is a stable isotope labeled derivative of 5-fluorouracil (5-FU), a commonly used chemotherapeutic agent in the treatment of various cancers, including breast, colorectal, and gastrointestinal cancers. 5-FU-6-d1 is used as a tracer in pharmacokinetic and clinical studies to determine the distribution, metabolism, and elimination of 5-FU in the body. The chemical structure of 5-FU-6-d1 is similar to that of 5-FU, with the exception of a deuterium atom at position 6 of the pyrimidine ring. This substitution enhances the stability of the molecule and allows for accurate measurement of 5-FU levels in biological fluids and tissues using mass spectrometry. 5-FU is a prodrug that requires conversion to its active metabolites, primarily 5-fluoro-2'-deoxyuridine monophosphate (FdUMP), to exert its cytotoxic effects. FdUMP inhibits thymidylate synthase, an enzyme required for DNA synthesis, leading to DNA damage and cell death. 5-FU-6-d1 undergoes the same metabolic transformations as 5-FU, but its deuterium label allows for more precise determination of the pharmacokinetic properties of the drug. In conclusion, 5-FU-6-d1 is a valuable tool for studying the pharmacokinetics and metabolism of 5-FU in the body. Its stable isotope label allows for accurate measurement of drug levels, enabling researchers to optimize dosing regimens and improve treatment outcomes for cancer patients.