4-β-Hydroxy Cholesterol-D7

4-β-Hydroxy Cholesterol-D7 is a stable isotope-labeled derivative of 4-β-hydroxycholesterol, a naturally occurring oxysterol synthesized in the liver from cholesterol. This compound has been identified as an endogenous ligand for the liver X receptors (LXRs), a class of transcription factors that regulate the expression of genes involved in cholesterol metabolism, inflammation, and immunity. As a result, 4-β-hydroxycholesterol has been implicated in the regulation of lipid homeostasis, atherosclerosis, and autoimmune disorders. The labeled version, 4-β-Hydroxy Cholesterol-D7, is used in research to study the metabolism and function of 4-β-hydroxycholesterol in vivo. It is commonly used as an internal standard in LC-MS/MS analysis to quantify endogenous levels of 4-β-hydroxycholesterol in biological samples, as well as to investigate its pharmacokinetics and tissue distribution in animal models. From a chemical perspective, 4-β-Hydroxy Cholesterol-D7 has a molecular formula of C27H43D7O2 and a molecular weight of 421.73 g/mol. It is a white to off-white powder that is soluble in organic solvents such as methanol, ethanol, and chloroform. The compound is stable under normal laboratory conditions and can be stored at -20°C for long periods of time without degradation. Overall, 4-β-Hydroxy Cholesterol-D7 is an important tool for investigating the role of 4-β-hydroxycholesterol in health and disease.