[3' 13C]Uridine

[3' 13C]Uridine is a modified form of uridine that has a carbon-13 isotope attached at the 3' position of the ribose sugar. Uridine itself is a nucleoside composed of uracil and ribose sugar and is an essential building block of RNA. [3' 13C]Uridine is widely used in metabolic labeling studies to track the biosynthesis of RNA in cells. The labeled carbon-13 atom at the 3' position of the ribose sugar is readily incorporated into newly synthesized RNA molecules and can be tracked using various analytical techniques, including nuclear magnetic resonance spectroscopy (NMR) and mass spectrometry. The use of [3' 13C]Uridine in metabolic labeling studies has provided valuable insights into the regulation of RNA synthesis and processing in various cellular processes, including gene expression, RNA splicing, and RNA turnover. The labeled nucleoside has also been used to investigate the dynamics of RNA metabolism in various disease states, including cancer and viral infections. The chemical properties of [3' 13C]Uridine are similar to those of natural uridine, which means that it can be easily incorporated into RNA molecules without affecting their structure or function. The labeled nucleoside is commercially available from various chemical suppliers and is typically supplied as a white powder or as a solution in water or organic solvents. Overall, [3' 13C]Uridine is a valuable tool for investigating the biosynthesis and turnover of RNA in cells and has numerous applications in basic research and drug discovery.