Ammonium-15N Acetate

Ammonium-15N acetate is a stable isotope-labeled compound that is commonly used in biochemical and biomedical research. It contains a nitrogen isotope, 15N, which is heavier than the naturally occurring 14N isotope. The compound is primarily used in metabolic studies to track the fate of nitrogen in biological systems. Ammonium-15N acetate is often administered to test subjects and incorporated into biological molecules such as proteins and nucleic acids. By analyzing the isotopic composition of these molecules, researchers can gain insight into metabolic pathways, protein turnover rates, and other important biological processes. Additionally, Ammonium-15N acetate can be used in mass spectrometry-based proteomics to identify and quantify proteins in complex samples. The chemical formula for Ammonium-15N acetate is CH3CO2NH4. It has a molecular weight of 77.08 g/mol and a melting point of 114-116 °C. The compound is soluble in water and has a slightly acidic pH. It is stable under normal conditions and can be stored long-term in a dry, cool place. In summary, Ammonium-15N acetate is a valuable tool for biologists and biochemists studying nitrogen metabolism and related processes. Its stable isotope labeling allows for precise measurements and analysis, making it a versatile and important compound in modern research.