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2-Hydroxybenzimidazole-d4 is a deuterated form of 2-hydroxybenzimidazole, a heterocyclic organic compound with the molecular formula C7H5NO. This isotopically labeled compound contains four deuterium atoms, which are heavier isotopes of hydrogen, and can be used in various chemical and biochemical research applications.
2-Hydroxybenzimidazole-d4 is commonly used as a stable isotope-labeled internal standard in mass spectrometry-based analyses. It can be used to quantify the levels of unlabeled 2-hydroxybenzimidazole in various samples. Additionally, it can be used in experiments where a deuterated compound is needed as a tracer to study the metabolic pathways of 2-hydroxybenzimidazole in living organisms.
In terms of its chemical properties, 2-Hydroxybenzimidazole-d4 is a white or off-white crystalline powder that is soluble in water, ethanol, and other polar solvents. It has a melting point of 300-305°C and a boiling point of 346°C. Its chemical structure contains a benzene ring fused to an imidazole ring, with a hydroxyl group attached to the benzene ring.
Overall, 2-Hydroxybenzimidazole-d4 is a useful compound for various research applications, particularly in the field of mass spectrometry-based analyses. Its deuterated form provides greater accuracy and precision in quantitative analyses, making it a valuable tool for researchers studying the metabolism and biological effects of 2-hydroxybenzimidazole.
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