a,4-Di-O-acetyl Vanillylmandelic Acid-d3 Ethyl Ester

4-Di-O-acetyl Vanillylmandelic Acid-d3 Ethyl Ester is a deuterated derivative of Vanillylmandelic Acid (VMA) and is used in biochemical research as a stable isotopic internal standard for the quantitative determination of VMA in biological fluids. VMA is a metabolite of the neurotransmitter epinephrine and norepinephrine and is used as a biomarker for the diagnosis of neuroblastoma, a type of cancer that develops from immature nerve cells. When VMA is present in biological samples, it can be extracted and purified, and then measured using liquid chromatography-mass spectrometry (LC-MS) or gas chromatography-mass spectrometry (GC-MS). However, the accuracy of the measurement can be affected by variations in extraction and purification efficiency, as well as by the presence of natural isotopes of VMA. By using 4-Di-O-acetyl Vanillylmandelic Acid-d3 Ethyl Ester as an internal standard, the variations in extraction and purification efficiency can be normalized, and the natural isotopes of VMA can be distinguished from the deuterated internal standard. The chemical structure of 4-Di-O-acetyl Vanillylmandelic Acid-d3 Ethyl Ester contains two acetyl groups and an ethyl ester group, in addition to the deuterated vanillyl and mandelic acid moieties. These groups increase the stability and solubility of the compound, and also provide additional functional groups for derivatization and labeling.