6-N-Acetyl-L-lysine-d8

6-N-Acetyl-L-lysine-d8 is a stable isotope-labeled analog of 6-N-acetyl-L-lysine, an acetylated derivative of the essential amino acid lysine. This compound is commonly used as an internal standard in mass spectrometry-based proteomics research to accurately quantify the levels of acetylated lysine residues in proteins. The chemical formula of 6-N-Acetyl-L-lysine-d8 is C9H18D8N2O3, and its molecular weight is 215.33 g/mol. This compound has eight deuterium atoms substituted for eight hydrogen atoms, which provides a clear distinction between the labeled and unlabeled forms of the molecule in mass spectrometry experiments. In terms of usage, 6-N-Acetyl-L-lysine-d8 can be added to protein samples prior to enzymatic digestion and mass spectrometry analysis. By comparing the peak intensities of the labeled and unlabeled forms of the acetylated lysine residues, researchers can accurately quantify the extent of lysine acetylation in the protein sample. Overall, 6-N-Acetyl-L-lysine-d8 is a valuable tool for studying lysine acetylation in proteomics research, and its stable isotope labeling properties make it a reliable internal standard for accurate quantification of acetylation levels.