17α-Estradiol-2,4-d2

17α-Estradiol-2,4-d2 is a synthetic form of the female hormone estradiol that contains two deuterium atoms at positions 2 and 4 of the molecule. It is commonly used in research as a stable isotope-labeled internal standard for the quantification of endogenous estradiol levels in biological samples. Estradiol plays a crucial role in the regulation of the female reproductive system, and aberrant levels of estradiol have been associated with a range of diseases including breast cancer, endometriosis, and osteoporosis. Accurate measurement of estradiol levels in biological samples is therefore important for both research and clinical purposes. The use of 17α-Estradiol-2,4-d2 as an internal standard allows for the accurate and precise measurement of endogenous estradiol levels in a variety of biological matrices including blood, urine, and tissue samples. It is typically used in conjunction with liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods for the quantification of estradiol. Chemically, 17α-Estradiol-2,4-d2 is a steroid hormone that is structurally similar to testosterone and progesterone. It acts by binding to estrogen receptors in target tissues, regulating gene expression and cellular function. While it is not approved for use in humans, it is widely used in research to further our understanding of the role of estradiol in health and disease.