4-Cholesten-3-one-2,2,4,6,6-d5

4-Cholesten-3-one-2,2,4,6,6-d5 is a deuterated form of 4-Cholesten-3-one, a steroid molecule that is found in the human body and is involved in the biosynthesis of hormones such as testosterone and estrogen. The deuterated form contains five deuterium atoms, which replace five of the six hydrogen atoms in the molecule. This isotopic substitution can be useful in a variety of scientific applications, including pharmacokinetic and metabolic studies. The chemical formula of 4-Cholesten-3-one-2,2,4,6,6-d5 is C27H35D5O, and its molecular weight is 397.67 g/mol. The compound is a white to off-white powder that is soluble in organic solvents such as chloroform and ethanol. It has a melting point of 130-132 °C. In research, 4-Cholesten-3-one-2,2,4,6,6-d5 is commonly used as a stable isotope-labeled internal standard in liquid chromatography-mass spectrometry (LC-MS) analyses. This technique can be used to quantitatively measure the levels of endogenous 4-Cholesten-3-one in biological samples, and to study its metabolism and distribution in the body. Overall, 4-Cholesten-3-one-2,2,4,6,6-d5 is a valuable tool in biomedical research, allowing for precise and accurate measurements of endogenous steroid molecules in complex biological systems.