5-Ethyl-2'-deoxyuridine-d5

5-Ethyl-2'-deoxyuridine-d5 is a stable isotope-labeled analog of 5-Ethyl-2'-deoxyuridine (EdU), which is a thymidine analog that is used in molecular biology to detect DNA synthesis. This compound contains five deuterium atoms at specific positions, which allows it to be distinguished from the natural EdU compound in mass spectrometry analysis. The primary usage of 5-Ethyl-2'-deoxyuridine-d5 is in cell proliferation assays, where it is incorporated into newly synthesized DNA during the S-phase of the cell cycle. By detecting the presence of EdU in the DNA using specific antibodies or fluorescent dyes, researchers can measure the rate of DNA synthesis and cell proliferation in a population of cells. This technique is widely used in cancer research, drug discovery, and cell biology studies. From a chemical perspective, 5-Ethyl-2'-deoxyuridine-d5 is a nucleoside analog that mimics the natural nucleoside thymidine in DNA. It is structurally similar to EdU, but the incorporation of deuterium atoms alters its physical and chemical properties. 5-Ethyl-2'-deoxyuridine-d5 is stable under normal laboratory conditions and can be stored for long periods of time without degradation. It is soluble in water and other polar solvents, making it easy to work with in biological assays. In conclusion, 5-Ethyl-2'-deoxyuridine-d5 is a useful tool for studying cell proliferation and DNA synthesis. Its stable isotope label allows for accurate detection and quantification, while its chemical properties make it easy to use in a variety of experimental settings.