2-Deoxy-D-glucose-13C6

2-Deoxy-D-glucose-13C6 (2DG-13C6) is a stable isotope-labeled form of 2-deoxy-D-glucose, which is a glucose analog that has been widely used as a metabolic tracer and inhibitor of glucose metabolism. 2DG-13C6 is used in metabolic studies to investigate glucose metabolism and its regulation in various biological systems, including cells, tissues, and animals. In terms of its chemical information, 2DG-13C6 is a synthetic compound with a molecular formula of C6H12O5 and a molecular weight of 180.16 g/mol. It is a white to off-white powder that is soluble in water and other polar solvents. The compound contains six carbon-13 (13C) isotopes, which can be detected and quantified by nuclear magnetic resonance (NMR) spectroscopy. The usage of 2DG-13C6 involves its incorporation into glucose metabolism pathways, where it competes with glucose for uptake and phosphorylation by the hexokinase enzyme, resulting in the inhibition of glycolysis and the accumulation of 2DG-13C6 in the cells. This metabolic trapping of 2DG-13C6 allows for the measurement of glucose uptake and utilization rates, as well as the detection of metabolic changes in response to various stimuli, such as insulin, hypoxia, or cancer. In conclusion, 2DG-13C6 is a valuable tool for investigating glucose metabolism and its regulation in biological systems. Its stable isotope labeling and metabolic trapping properties allow for accurate and sensitive measurements of glucose metabolism rates and pathways, which can provide insights into various physiological and pathological processes.