5'-Deoxy-5'-(methylthio)adenosine-13C

5'-Deoxy-5'-(methylthio)adenosine-13C is a modified form of adenosine, a nucleoside that plays a crucial role in various biological processes including energy metabolism, signal transduction, and gene expression. This modified compound is labeled with a stable isotope, 13C, which enhances its usefulness in many biochemical and biological research applications. One of the most common uses of 5'-Deoxy-5'-(methylthio)adenosine-13C is in metabolic labeling experiments. This compound is often used as a tracer to study the biosynthesis and metabolism of adenosine in cells and tissues. The labeled compound is added to the growth medium of cells, and its incorporation into cellular nucleotides is monitored using various analytical techniques such as high-performance liquid chromatography (HPLC) and mass spectrometry (MS). In addition to metabolic labeling studies, 5'-Deoxy-5'-(methylthio)adenosine-13C can also be used in other types of research applications such as drug discovery, enzymology, and structural biology. This compound is a potent inhibitor of various enzymes that are involved in adenosine metabolism, such as adenosine deaminase and purine nucleoside phosphorylase. Therefore, it can be used to study the functions and mechanisms of these enzymes in vitro and in vivo. In terms of its chemical properties, 5'-Deoxy-5'-(methylthio)adenosine-13C is a stable and water-soluble compound that can be easily synthesized from commercially available starting materials. It has a molecular weight of 330.3 g/mol and a chemical formula of C11H14N5O3S. Its structure consists of a purine base (adenine) attached to a ribose sugar moiety through a glycosidic bond. The 5'-hydroxyl group of the ribose is replaced by a methylthio group in this modified compound.