5-Fluorouridine-13C,15N2

5-Fluorouridine-13C,15N2, also known as 5-FUrd, is a stable, isotopically labeled analog of the nucleoside uridine. It is commonly used in biological research as a tracer molecule to study the metabolism and kinetics of uridine in cells and tissues. The labeling of 5-FUrd with carbon-13 and nitrogen-15 isotopes provides a distinct molecular signature that can be tracked using mass spectrometry. In addition to its use as a tracer, 5-FUrd has also been investigated for its potential as an antineoplastic agent. 5-FUrd is converted to 5-fluorouracil (5-FU) by the enzyme thymidine phosphorylase, which is overexpressed in some types of cancer cells. 5-FU is an antimetabolite that inhibits DNA synthesis and cell division, and is used clinically in the treatment of various types of cancer, including breast, colon, and stomach cancers. The isotopically labeled 5-FUrd can be used to study the pharmacokinetics and tissue distribution of 5-FU in vivo. Some caution should be exercised when handling 5-FUrd, as it is a toxic substance. It should be stored in a cool, dry place and handled with appropriate protective equipment. Overall, 5-FUrd is a useful tool for studying the metabolism and therapeutic potential of uridine and 5-FU in biological systems.