5-Aza Cytosine-15N4

5-Aza Cytosine-15N4 is a synthetic nucleoside analog of cytosine that is used in a variety of research applications. It is commonly used in epigenetic studies as a demethylating agent, where it is used to reverse the hypermethylation of DNA that can occur in cancer cells. This compound is also used in the study of DNA replication and repair, as well as in the development of new cancer treatments. Chemically, 5-Aza Cytosine-15N4 is a modified version of cytosine, in which one of the nitrogen atoms has been replaced with a heavier isotope, nitrogen-15. This modification allows researchers to track the movement and incorporation of the compound into DNA and RNA molecules using mass spectrometry techniques. In addition to its research applications, 5-Aza Cytosine-15N4 has shown promise as a potential therapeutic agent for the treatment of cancer. It works by inhibiting the activity of DNA methyltransferase enzymes, which are responsible for adding methyl groups to DNA. By inhibiting this activity, 5-Aza Cytosine-15N4 can help to reactivate tumor-suppressor genes that have been silenced by hypermethylation, leading to the death of cancer cells. Overall, 5-Aza Cytosine-15N4 is a valuable tool in the fields of epigenetics and cancer research, with the potential to lead to new treatments for a variety of diseases.