5-Aza-2’-deoxy Cytidine-15N4

5-Aza-2’-deoxy Cytidine-15N4 is a chemical compound with the molecular formula C9H12N4O4 and a molecular weight of 252.22 g/mol. It is a stable isotope-labeled form of 5-Aza-2’-deoxy Cytidine, which is a potent inhibitor of DNA methyltransferase and has been used as an anti-cancer agent. The usage of 5-Aza-2’-deoxy Cytidine-15N4 is mainly in research studies related to DNA methylation, epigenetics, and cancer biology. It can be used as a tracer in isotope dilution mass spectrometry for the quantification of DNA methylation levels. Additionally, 5-Aza-2’-deoxy Cytidine-15N4 can also be used as a tool for studying the role of DNA demethylation in cancer progression and for evaluating the efficacy of anti-cancer drugs targeting DNA methylation. In terms of chemical information, 5-Aza-2’-deoxy Cytidine-15N4 is a nucleoside analogue that incorporates into DNA during replication, leading to the inhibition of DNA methyltransferase and subsequent hypomethylation of DNA. This can result in the reactivation of tumor suppressor genes that were previously silenced by DNA methylation, leading to the induction of apoptosis and inhibition of cancer cell growth. In conclusion, 5-Aza-2’-deoxy Cytidine-15N4 is a valuable tool in research studies related to DNA methylation, epigenetics, and cancer biology. Its usage as a tracer in isotope dilution mass spectrometry and as a tool for studying the role of DNA demethylation in cancer progression highlights its importance in the field of cancer research.