Physostigmine is a medication used to treat certain medical conditions, including glaucoma and myasthenia gravis. However, it is important to ensure that the physostigmine used is pure, as impurities can potentially cause harmful side effects. Various impurities can be introduced during the production process or due to environmental factors, such as exposure to high temperatures or humidity. These impurities may include degradation products, residual solvents, or other organic and inorganic impurities. Therefore, it is crucial to carefully identify and monitor impurities in physostigmine to ensure its safety and efficacy.