Sancycline is a broad-spectrum antibiotic used to treat various infections caused by Gram-positive and Gram-negative bacteria. However, during the manufacturing process, impurities may be introduced that can affect the purity and potency of the final product. Sancycline impurities can include degradation products, residual solvents, and other impurities that need to be carefully monitored and controlled to ensure the safety and efficacy of the antibiotic. Analytical techniques such as HPLC and GC are used to detect and quantify these impurities.