Zidovudine, also known as AZT, is an antiretroviral medication used in the treatment of HIV/AIDS. However, during its manufacturing process, various impurities may be formed, which can affect the quality and efficacy of the drug. These impurities include related substances, degradation products, and residual solvents, which must be identified, quantified, and controlled to ensure the safety and effectiveness of the medication. Analytical methods such as high-performance liquid chromatography (HPLC) and mass spectrometry (MS) are used to detect and measure these impurities in pharmaceutical products.