[2-13C,15N2]-Uracil

[2-13C,15N2]-Uracil is a modified form of uracil, a nitrogenous base that is commonly found in RNA molecules. This modified version of uracil contains two carbon-13 atoms and two nitrogen-15 atoms, which are stable isotopes of the corresponding elements. The presence of these stable isotopes makes [2-13C,15N2]-Uracil useful in various scientific applications, particularly in studies of nucleic acids and metabolic pathways. One of the primary uses of [2-13C,15N2]-Uracil is in stable isotope labeling experiments. In these experiments, scientists replace the natural uracil in a sample with [2-13C,15N2]-Uracil, which is then incorporated into newly synthesized RNA molecules. By analyzing the isotopic composition of the resulting RNA, researchers can gain insights into various cellular processes, such as gene expression and protein synthesis. In addition to its uses in stable isotope labeling experiments, [2-13C,15N2]-Uracil can also be used as a metabolic tracer. When added to a cell culture or organism, the labeled uracil is taken up and metabolized, allowing researchers to track the fate of the labeled atoms in various metabolic pathways. Overall, [2-13C,15N2]-Uracil is a valuable tool for studying nucleic acids and cellular metabolism. Its unique isotopic composition allows for precise measurements and insights into complex biological processes.