3,5-Dinitrosalicylhydrazide-15N2

3,5-Dinitrosalicylhydrazide-15N2, also known as DNSH-15N2, is a chemical compound that is widely used in analytical chemistry applications as a reagent for the quantification of carbonyl groups in proteins and other biomolecules. This compound is a stable and non-toxic derivative of hydrazine that reacts with carbonyl groups to form a stable Schiff base, which can be detected spectrophotometrically at 540 nm. The use of DNSH-15N2 in carbonyl quantification assays has become a common practice in biochemical and biomedical research. The assay is a reliable way to measure oxidative damage to proteins, which is linked to various age-related diseases and pathologies. The DNSH-15N2 assay can also be used to detect oxidative stress in cells, tissues, and organisms, providing insights into cellular and organismal health. Chemically, DNSH-15N2 is a derivative of dinitrosalicylic acid, which is a nitrophenol compound. The 15N2 in the compound name denotes the presence of two nitrogen atoms with isotopic mass 15, which is useful for isotopic labeling studies. DNSH-15N2 has a molecular weight of 212.14 g/mol and a melting point of 230-232°C. It is soluble in water, methanol, and ethanol but insoluble in most organic solvents. In conclusion, DNSH-15N2 is a useful reagent for carbonyl quantification assays in biochemical and biomedical research. Its stable and non-toxic nature makes it an attractive alternative to other reagents. Its chemical properties make it suitable for isotopic labeling studies as well.