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3,5-Dinitrosalicylic acid (DNS) is a commonly used reagent in biochemistry for the detection and quantification of reducing sugars. The compound is used to measure the presence of reducing sugars, such as glucose, fructose, and lactose, in a variety of biological samples. The 13C6 isotopically labeled version of DNS is used for research purposes to determine the fate of labeled glucose in metabolic pathways.
In the presence of reducing sugars, DNS undergoes a chemical reaction that produces a colored product. The reaction involves the reduction of the nitro groups in DNS by the reducing sugar, resulting in the formation of 3-amino-5-nitrosalicylic acid. The intensity of the color produced is directly proportional to the amount of reducing sugar present in the sample.
The chemical formula of 3,5-Dinitrosalicylic-13C6 acid is C7H3NO7^13C6. It has a molar mass of 239.11 g/mol. The isotopic labeling of the compound allows for the tracking of labeled glucose in metabolic pathways through techniques such as mass spectrometry. The compound is stable under normal laboratory conditions and can be stored for extended periods of time.
3,5-Dinitrosalicylic-13C6 acid is an important tool in biochemistry research for the detection and quantification of reducing sugars and the tracking of labeled glucose in metabolic pathways. Its stability and ease of use make it a popular choice for researchers in the field.
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