5,6-Dihydrouracil D4

5,6-Dihydrouracil D4 is a deuterated form of 5,6-dihydrouracil, a derivative of uracil. It is a white crystalline powder with a molecular formula of C4H2D4N2O2 and a molecular weight of 120.16 g/mol. This compound is primarily used as a stable isotope-labeled internal standard for mass spectrometry analysis of nucleosides and nucleotides. 5,6-Dihydrouracil D4 is a key compound in the biosynthesis of thymine, a building block of DNA. It is also a metabolite of uracil, which is produced during the breakdown of RNA and DNA. The deuterated form of this compound is useful in mass spectrometry analysis as it allows for accurate quantification of nucleosides and nucleotides in complex biological samples. This compound is stable under normal conditions and has a melting point of 215-218 °C. It is soluble in water, ethanol, and dimethyl sulfoxide (DMSO). However, it is insoluble in most organic solvents. Overall, 5,6-Dihydrouracil D4 is an important compound in the field of biochemistry and mass spectrometry analysis. Its stable isotope-labeled form allows for accurate and reliable quantification of nucleosides and nucleotides, making it a valuable tool for researchers studying DNA and RNA metabolism.