Ethyl 3-(3-amino-4-(methyl(nitroso)amino)-N-(pyridin-2-yl)benzamido)propanoate

Ethyl 3-(3-amino-4-(methyl(nitroso)amino)-N-(pyridin-2-yl)benzamido)propanoate, commonly known as MNAE, is a chemical compound with a molecular formula of C20H22N6O6. It is a yellow crystalline powder that is soluble in organic solvents such as dimethyl sulfoxide and dimethylformamide. MNAE is commonly used as a fluorescent probe in biochemical studies, specifically in the study of protein-protein interactions. Its unique structure allows it to bind to proteins and emit fluorescence upon excitation, allowing researchers to visualize protein-protein interactions in real-time. In addition to its use in biochemical studies, MNAE has also been studied for its potential anti-tumor properties. Research has shown that MNAE can induce apoptosis (programmed cell death) in cancer cells, making it a potential candidate for future cancer therapies. However, it is important to note that MNAE is a potentially hazardous chemical and should only be handled by trained professionals. It is classified as a hazardous material by the United States Department of Transportation and can cause severe skin and eye irritation if not handled properly. It is also important to use protective equipment, such as gloves and goggles, when working with MNAE.