Isopropyl -β-D-Thiogalactopyranoside, Dioxane Free

Isopropyl-β-D-Thiogalactopyranoside or IPTG is a chemical compound commonly used in molecular biology experiments as an inducer of gene expression in bacterial cells. This chemical compound is a synthetic analog of lactose and is used to activate the lac operon in Escherichia coli (E.coli) bacterial cells. IPTG is commonly used in genetic engineering and recombinant DNA technology to induce the expression of cloned genes in bacterial cells. IPTG is a non-toxic and non-mutagenic compound that is soluble in water and ethanol. It is commonly used in concentrations ranging from 0.1 mM to 10 mM, depending on the experimental setup. The dioxane-free variant of IPTG is preferred in molecular biology experiments as dioxane is a toxic and carcinogenic solvent that can interfere with the results of experiments. IPTG works by binding to the lac repressor protein, which normally binds to the operator region of the lac operon and inhibits gene expression. When IPTG is added to the bacterial culture, it binds to the lac repressor protein and causes a conformational change, resulting in the release of the repressor from the operator region. This allows RNA polymerase to transcribe the genes of interest, resulting in the production of proteins. In conclusion, IPTG is a commonly used chemical compound in molecular biology experiments to induce gene expression in bacterial cells. Its non-toxic and non-mutagenic properties make it a safe and reliable chemical for use in research laboratories. The dioxane-free variant of IPTG is preferred for use in experiments to avoid potential interference from toxic solvents.