2-DEOXYCYTIDINE PHOSPHORAMIDITE 15N3

2-Deoxycytidine phosphoramidite 15N3 is a modified nucleotide used in the synthesis of DNA and RNA molecules. It is commonly used in research and pharmaceutical industries as a tool for studying DNA replication, transcription, and translation. The 15N3 label indicates that three nitrogen atoms in the molecule have been replaced with the stable isotope nitrogen-15, which makes it useful in nuclear magnetic resonance (NMR) spectroscopy studies. The chemical formula of 2-Deoxycytidine phosphoramidite 15N3 is C18H25N7O7P and its molecular weight is 523.42 g/mol. It is a white crystalline powder that is soluble in organic solvents such as acetonitrile and dichloromethane. The compound reacts with other nucleotide monomers to form phosphodiester bonds and create long chains of DNA or RNA. The usage of 2-Deoxycytidine phosphoramidite 15N3 requires expertise in synthetic chemistry and molecular biology. It is commonly used in the synthesis of oligonucleotides, which are short DNA or RNA sequences that can be used as probes or primers for various applications such as gene expression analysis, genetic engineering, and disease diagnosis. The labeled nucleotide is incorporated into the oligonucleotide using automated DNA synthesizers and purified using high-performance liquid chromatography (HPLC) techniques. In conclusion, 2-Deoxycytidine phosphoramidite 15N3 is a valuable tool in molecular biology research and drug development. Its stable isotope label and ability to form phosphodiester bonds make it an essential component of synthetic oligonucleotide synthesis. Its usage requires specialized knowledge and expertise in synthetic chemistry and molecular biology.